aly mice Search Results


mouse alyref  (OriGene)
90
OriGene mouse alyref
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Mouse Alyref, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pmc07307164-291-2-15?v=OriGene
Average 90 stars, based on 1 article reviews
mouse alyref - by Bioz Stars, 2026-07
90/100 stars
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myc map3k14  (OriGene)
90
OriGene myc map3k14
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Myc Map3k14, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pmc03536768-166-9-11?v=OriGene
Average 90 stars, based on 1 article reviews
myc map3k14 - by Bioz Stars, 2026-07
90/100 stars
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mutant homozygous aly/aly control wild-type aly/þ mice  (clea japan inc)
90
clea japan inc mutant homozygous aly/aly control wild-type aly/þ mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Mutant Homozygous Aly/Aly Control Wild Type Aly/þ Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm16640660-32-7-11?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
mutant homozygous aly/aly control wild-type aly/þ mice - by Bioz Stars, 2026-07
90/100 stars
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aly/aly jcl mutant mice  (clea japan inc)
90
clea japan inc aly/aly jcl mutant mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Aly/Aly Jcl Mutant Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm15699117-40-9-21?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
aly/aly jcl mutant mice - by Bioz Stars, 2026-07
90/100 stars
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alymphoplasia mice map3k14 / , thy1.2, h-2b; hereafter aly/aly  (clea japan inc)
90
clea japan inc alymphoplasia mice map3k14 / , thy1.2, h-2b; hereafter aly/aly
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Alymphoplasia Mice Map3k14 / , Thy1.2, H 2b; Hereafter Aly/Aly, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm16547240-56-0-12?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
alymphoplasia mice map3k14 / , thy1.2, h-2b; hereafter aly/aly - by Bioz Stars, 2026-07
90/100 stars
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b6-aly/+ mice  (clea japan inc)
90
clea japan inc b6-aly/+ mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
B6 Aly/+ Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm09450244-24-4-11?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
b6-aly/+ mice - by Bioz Stars, 2026-07
90/100 stars
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nik aly mice (aly/nsc jcl aly/aly)  (clea japan inc)
90
clea japan inc nik aly mice (aly/nsc jcl aly/aly)
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Nik Aly Mice (Aly/Nsc Jcl Aly/Aly), supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm26116508-50-0-9?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
nik aly mice (aly/nsc jcl aly/aly) - by Bioz Stars, 2026-07
90/100 stars
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female aly® nscjcl aly/aly and aly/+ mice  (clea japan inc)
90
clea japan inc female aly® nscjcl aly/aly and aly/+ mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Female Aly® Nscjcl Aly/Aly And Aly/+ Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pmc03424244-42-5-15?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
female aly® nscjcl aly/aly and aly/+ mice - by Bioz Stars, 2026-07
90/100 stars
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specific pathogen-free, male mutant homozygous aly/aly and control wild-type aly/θ mice  (clea japan inc)
90
clea japan inc specific pathogen-free, male mutant homozygous aly/aly and control wild-type aly/θ mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Specific Pathogen Free, Male Mutant Homozygous Aly/Aly And Control Wild Type Aly/θ Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm16640660-32-1-11?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
specific pathogen-free, male mutant homozygous aly/aly and control wild-type aly/θ mice - by Bioz Stars, 2026-07
90/100 stars
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b6 (h-2b) mice homozygous for the mutation that leads to alymphoplasia (aly)  (clea japan inc)
90
clea japan inc b6 (h-2b) mice homozygous for the mutation that leads to alymphoplasia (aly)
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
B6 (H 2b) Mice Homozygous For The Mutation That Leads To Alymphoplasia (Aly), supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm15378574-20-6-15?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
b6 (h-2b) mice homozygous for the mutation that leads to alymphoplasia (aly) - by Bioz Stars, 2026-07
90/100 stars
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aly/aly homozygous and aly/+ heterozygous mice  (clea japan inc)
90
clea japan inc aly/aly homozygous and aly/+ heterozygous mice
The m5C reader <t>ALYREF</t> regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.
Aly/Aly Homozygous And Aly/+ Heterozygous Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aly+mice/pm16303288-47-4-10?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
aly/aly homozygous and aly/+ heterozygous mice - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


The m5C reader ALYREF regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.

Journal: Journal of Virology

Article Title: 5-Methylcytosine RNA Modifications Promote Retrovirus Replication in an ALYREF Reader Protein-Dependent Manner

doi: 10.1128/JVI.00544-20

Figure Lengend Snippet: The m5C reader ALYREF regulates MLV production. (A) Effect of ALYREF knockdown on cellular MLV protein levels. After transfection of 3T3 cells with siRNAs targeting ALYREF or nontargeting (NT) siRNAs, cells were infected with MLV for 24 h; after an additional 48 h of incubation, cellular proteins and media were collected. A representative Western blot of MLV capsid protein (CA) levels from infected 3T3 cells following siRNA-mediated knockdown of ALYREF is shown, with GAPDH as a loading control. (B) Quantitative analysis of MLV CA levels in cellular lysates upon ALYREF depletion. Data are means plus SEM for 3 biological replicates; **, P ≤ 0.01. (C) Quantitative analysis of MLV release upon ALYREF knockdown, as determined by MLV capsid levels from harvested medium samples. Data are means plus SEM for 6 biological replicates; **, P ≤ 0.01. (D) Quantitative analysis of MLV CA levels upon ALYREF overexpression as determined by MLV capsid levels in cellular lysates, normalized to GAPDH. Data are means plus SEM for 3 biological replicates. (E) Rescue of ALYREF depletion. 3T3 cells were transfected with an siRNA targeting the 3′ UTR of ALYREF. After 24 h, cells were cotransfected with a vector for wild-type MLV (pNCA) and a plasmid encoding either wild-type ALYREF (pALYREF) or an ALYREF mutant with defective m5C binding (pALYREF-K171A) or a control vector. MLV CA levels were quantified and normalized to the GAPDH loading control. Data are means plus SEM for 2 biological replicates.

Article Snippet: Plasmids encoding mouse ALYREF (catalog no. MR220236) and NSUN2 (catalog no. MR208356) were purchased from OriGene, as well as a control vector (catalog no. PS100001).

Techniques: Knockdown, Transfection, Infection, Incubation, Western Blot, Control, Over Expression, Plasmid Preparation, Mutagenesis, Binding Assay